Video Capture and Editing microscopy allows fast and informative vouchering of individual nematodes prior to DNA amplification

Video Capture and Editing microscopy allows fast and informative vouchering of individual nematodes and other meiofauna prior to DNA amplification and many other destructive methods

Outline

Prologue

Prologue

The core idea: Capture focusing action as a video clip

The core idea: Capture focusing action as a video clip

Many uses for research of fragile microscopic specimens

When do I catch up with technology (and part with my savings)?

Video capture is ready, affordable and relatively reliable

Mid-price solution: manual focus and analog capture on DIC scope

Unusual parts on the microscope

Unusual parts for the desktop
computer

More expensive and more standardized solution: motorized focus and digital capture

Next step: robotic microscope, HDV camcorders?

Capture morphology of live nematodes before PCR

Capture morphology of live nematodes before PCR

Works with other meiofauna too

Our preferred barcode in nematodes: D2D3

Example: inventory of marine nematodes from Gulf of California

Attempted integration with ecological studies: vernal pools

Attempted integration with ecological studies: vernal pools

Online database for depositing clips and sequences: www.nematol.unh

Philosophy: maximize retrievable data per specimen

Wider perspective: discovery process needs multiple strategies

Wider perspective: emergent network of databases and projects

With Thanks To